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myc ddk  (OriGene)


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    Structured Review

    OriGene myc ddk
    Myc Ddk, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+ddk/pCMV6-AC-Myc-DDK+Mammalian+Expression+Vector/pm42286213-266-5-9
    Average 94 stars, based on 16 article reviews
    myc ddk - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.
    Article Snippet: .. The PCR product was then digested with Sgr I and Mlu I and cloned into pCMV6- AN- Myc- DDK (PS100016, OriGene) at the Sgr I and Mlu Irestriction sites. ..

    Clone Assay:

    Article Title: Dicer is cleaved by the Leader protease encoded by foot-and-mouth disease virus to promote infection in mammalian cells.
    Article Snippet: .. The PCR product was then digested with Sgr I and Mlu I and cloned into pCMV6- AN- Myc- DDK (PS100016, OriGene) at the Sgr I and Mlu Irestriction sites. ..

    Article Title: ESCRT III Regulates Lysosomal Perinuclear Clustering by Inhibiting Kinesin 1 Leading to Agnps Cytotoxicity in Human Cells
    Article Snippet: .. For CHMP4B and Kinesin 1 over-expression, Myc-DDK-tagged human CHMP4B (Catalog#: RC207637, OriGene, Rockville, MD, USA) and Kinesin 1 full-length cDNA was cloned into pCDNA 3.1 plasmid. ..

    Over Expression:

    Article Title: LIPT1 loss confers replication stress and PARP inhibitor sensitivity through PrimPol-mediated ssDNA gaps
    Article Snippet: For α-KG rescue, cells were pretreated with 1 mM dimethyl-α-KG or α-KG (MilliporeSigma) for 24 hours before the indicated assays. .. For L2HGDH overexpression, H460 WT and LIPT1 −/− cells were transfected 24 to 48 hours before DNA fiber assays with L2HGDH plasmids tagged with either Myc-DDK or GFP (RG217631 and RC217631, OriGene) using Lipofectamine 3000 (L3000150, Invitrogen, Thermo Fisher Scientific, Waltham, MA). ..

    Transfection:

    Article Title: LIPT1 loss confers replication stress and PARP inhibitor sensitivity through PrimPol-mediated ssDNA gaps
    Article Snippet: For α-KG rescue, cells were pretreated with 1 mM dimethyl-α-KG or α-KG (MilliporeSigma) for 24 hours before the indicated assays. .. For L2HGDH overexpression, H460 WT and LIPT1 −/− cells were transfected 24 to 48 hours before DNA fiber assays with L2HGDH plasmids tagged with either Myc-DDK or GFP (RG217631 and RC217631, OriGene) using Lipofectamine 3000 (L3000150, Invitrogen, Thermo Fisher Scientific, Waltham, MA). ..

    Plasmid Preparation:

    Article Title: ESCRT III Regulates Lysosomal Perinuclear Clustering by Inhibiting Kinesin 1 Leading to Agnps Cytotoxicity in Human Cells
    Article Snippet: .. For CHMP4B and Kinesin 1 over-expression, Myc-DDK-tagged human CHMP4B (Catalog#: RC207637, OriGene, Rockville, MD, USA) and Kinesin 1 full-length cDNA was cloned into pCDNA 3.1 plasmid. ..

    Generated:

    Article Title: LRBA regulates actin cytoskeleton dynamics through NMIIA during B cell immune responses.
    Article Snippet: Images were acquired using a 20x objective with a confocal microscope (LSM710, Zeiss) and signal quantification was performed using Duolink Image Tool (Sigma-Aldrich). .. Human LRBA full-length tagged to Myc-DDK was purchased from Origene (pCMV6-AC-Myc-DDK-LRBA). pCMV-mCherry-MHCIIA, pcDNA3-myc-FLNa WT, EGFP-Vimentin-7 and mCherryITSN2 were purchased from Addgene. pcDNA3-HA-TPM3 and pcDNA3-Flag-MYO18A were generated by the Signaling Factory & Robotics facility at the University of Freiburg, Germany. .. Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega).



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